For my research project, I employed a high-throughput approach. In detail, I focused on genomic and transcriptomic analysis of patients who developed an extramedullary localization, showing a significant difference in gene expression between bone marrow and secondary sites samples. This result provided the rationale for a functional enrichment analysis, which revealed an up-regulation of pathways involved in systemic spreading of leukemic blasts. This finding was confirmed by migration and invasion functional assays, exploiting a cell-line modeling leukemia cell behavior.